Prequel bonus - Have a saved game file from Strong Bad's Cool Game For Attractive People: Episode 1 - Homestar Ruiner to have all costume pieces from that game.

Strong Bad's wardrobe items - Search the following areas to find the indicated wardrobe item:

Beret: Send The Cheat to an empty field in the Maps And Minions mini-game.
Camo Pants: Dryer in the basement of Strong Bad's house.
Crown: Unplug the KoT's computer.
Eye Patch: Cereal box in Strong Bad's house.
Lectric Boots: Send an e-mail requesting them from the KoT's form letter for deliveries.
KoT's Beard: Right side shelf of the KoT's throne.
Strong Badia Shirt: Dig to the left of Strong Badia with the Taranchula Heavy Metal Detector.

Flammable items - The following items can be set ablaze:

Bleak House Flag in Strong Sad's room, after escaping (required to progress).
Bleak House Constitution in Strong Sad's room, after escaping (required to progress).
Bleak House Bill of Rights in Strong Sad's room, after escaping (required to progress).
Smoke detector in the living room of Strong Bad's house.
Strong Bad's bed in Strong Bad's room.
Kimono on the wall inside Pompomerania/Club Technochocolate.
Paper mache skulls in country during Strong Mad sequence (required to progress).
Lonely skull in country near the flag.
Lonely skull in country near where Strong Mad starts.
Bush at the Homsar Reserve.
Candles in the KoT's castle.
UN model in Marzipan's backyard; after winning.
Board game in Strong Badia.

Flag locations - Search the following locations to find the indicated flag:

Strong Badia: Search the brick wall in the field.
The Cheat And Tirerea: Get The Cheat to join you, then check the Tire.
Concession-Stan : Search the bush on the right side of the Concession Stand.
Bleak House: Search the box on Strong Sad's bed.
Homsar Reservation: Search the "mysterious" bush near Homsar.
Peoples' Republic Of Marzistar: Use the Metal Detector to the right of the onion patch at Marzipan's.
Pompomerania: Get Pom Pom to join you, then go to the bar and order a can of Bull Honkey.
Country: Use the Metal Detector near Country's flag.

Game manual page locations - Search the following locations to find the indicated game manual page:

Search the snail-mailbox at the House of Strong.
Search under the package near the low white fence in the Field.
Search the mailbox at Marzistar.
Search under the box near the bar at In Club Technochocolate/Pompomerania.

TGS Idea locations - Search the following locations to find the indicated TGS Idea:

Search the bookcase in Strong Sad's room after escaping the house.
Use the lighter on the barred window in the House Of Strong.
Use the Metal Detector to the right of the cave at the Homsar Reservation.
Search the "ex-fish" three times at the KOT's castle.

Strong Sad name calling - Successfully complete the indicated task:

Talk to Strong Sad during Part 1.
Talk to Strong Sad about the invisible fence two times during Part 1.
Search the shower in the House of Strong.
Use the pillow on Strong Sad during Part 1.
Put the Pilot Wings medal on Strong Sad during Part 2.
Talk to Strong Sad about Strong Badia during in Part 2.
Talk to Strong Sad about Bleak House during Part 2.
Use the fake sword on Strong Sad in Bleak House.
Talk to Strong Sad about Strong Badia after burning all his items.
Use the tuning fork on Strong Sad at the Homsar Reservation.
Use the Fake Sword on Strong Sad while at the Homsar Reservation
Talk to Strong Sad at Marzipan's.
Successfully complete the game, then talk to Strong Sad in his room.

Characters at attention - Successfully complete the indicated task to get the corresponding character at attention:

Have the Cheat join you.
Talk to Coach Z while preparing for the castle battle.
Talk to Pom Pom while preparing for the castle battle.
Talk to Strong Sad while preparing for the castle battle.
Talk to Strong Mad while preparing for the castle battle.